Rapid characterization of analytes in saliva

A system rapidly detects analytes in saliva using a light source, sensor array, and detector, with chemically sensitive particles in cavities that signal interactions with analytes, characterized through pattern recognition techniques.

Background

Saliva has garnered significant interest as a diagnostic medium due to its non-invasive collection and the presence of biomarkers indicative of various diseases. However, the low concentration of relevant markers in saliva compared to blood presents a notable challenge, necessitating highly sensitive detection methods. Traditional approaches often require sophisticated instrumentation, lengthy assays, and substantial reagent quantities, limiting their practicality for rapid and widespread diagnostic use. Moreover, these methods typically focus on single-analyte detection, which is inefficient for comprehensive health assessments. The viscous nature of saliva further complicates automated fluid handling, often requiring significant dilution that can compromise assay sensitivity. Consequently, there is a pressing need for innovative solutions that can rapidly and accurately detect multiple analytes in saliva, overcoming the limitations of current diagnostic technologies.

Technology description

A system for the rapid characterization of analytes in saliva features a light source, a sensor array, and a detector. The sensor array is built on a supporting member with multiple cavities, each designed to hold specific chemically sensitive particles. These particles generate a signal when they interact with cardiovascular risk factor analytes, facilitated by a receptor coupled to the particle. The particle-analyte complex is then visualized using a reagent. Pattern recognition techniques are employed to characterize the analytes within a multi-analyte fluid. Additionally, flexible projections over each cavity ensure the retention of particles, allowing for precise and reliable detection.

What differentiates this technology is its ability to rapidly and accurately characterize multiple analytes simultaneously from a single saliva sample. The use of chemically sensitive particles and advanced pattern recognition techniques enables the detection of cardiovascular risk factors with high specificity. The design of the cavities, tailored to capture specific particle sizes, enhances precision. Furthermore, the flexible projections ensure that the particles remain securely in place, reducing the risk of error and improving the reliability of results. This combination of features makes the system a powerful tool for non-invasive medical diagnostics.