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Mutant TEV proteases for enhanced protein cleavage
This invention describes mutant TEV proteases with enhanced activity for cleaving specific amino acid sequences. These proteases are useful for removing purification tags from fusion proteins and antibodies in lab settings.
Background
Protein purification is a cornerstone of biological research and drug development. The ability to isolate and...
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Harnessing the Type III-Dv CRISPR-Cas system for targeted RNA modification and detection
The Type III-Dv CRISPR-Cas system is a genetic tool designed for precise RNA targeting and cleavage. It uses a complex of Cas proteins to bind and modify single-stranded nucleic acids, enabling applications in gene silencing and RNA detection.
Background
The field of CRISPR-Cas technology has revolutionized genetic engineering by providing precise...
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Modified CRISPR-Cas9 system for enhanced gene editing
This invention enhances the CRISPR-Cas9 gene-editing system by modifying the Cas9 protein’s Rec3 clamp region, improving its fidelity, specificity, and speed, reducing off-target effects, and increasing precision for therapeutic and research applications.
Background
Genome engineering is a rapidly advancing field that involves the precise modification...
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Catalytic systems for controlled ring-opening polymerization reactions
The invention describes catalyst systems for ring-opening polymerization of cyclic compounds like epoxides and lactones. These catalysts, using Group 13 and 15 metals, enable controlled polymerization, producing polymers with precise molecular weights and functionalities, and can extend existing polymers to form block copolymers.
Background
Ring-opening...
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Expression system for labeling and targeting cells with elevated calcium levels
The technology describes a system for labeling and targeting cells with elevated calcium levels, such as cancer cells or activated neurons, using a CREB reporter system. This system includes a synthetic CRE enhancer linked to promoters that control the expression of genes encoding functional proteins like reporters, ion channels, or therapeutic agents,...
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Bioinformatics-powered CRISPR-transposon systems for enhanced gene editing and horizontal gene transfer
CRISPR-associated transposons (CASTs) use nuclease-deficient CRISPR-Cas systems for RNA-guided gene insertion, enhancing the gene-editing toolkit by enabling precise DNA integration.
Background
CRISPR-associated transposons (CASTs) represent a significant advancement in gene-editing technology, originating from the fusion of CRISPR-Cas systems with...
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Processes and compositions to expand drug processing
Background
Dr. Robert O. Williams III and his team have developed a method to expand the ability of the hot-melt extrusion process to increase drug loading in amorphous solid dispersions (ASD). Dr. Williams is the Division Head and Professor of Molecular Pharmaceutics and Drug Delivery and the Johnson & Johnson Centennial Chair in Pharmacy at UT...
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An RNA identification beacon based on Cas12a2
Background
The CRISPR-Cas systems are adaptive immune mechanisms found in prokaryotes, primarily bacteria and archaea, which protect against invading genetic elements such as phages and plasmids. These systems have been harnessed for various biotechnological applications, including genome editing and molecular diagnostics. Cas12a2, a particular variant...
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Engineered RNA polymerases and capping enzymes for the expression of 7‑methylguanylate capped RNA
Background
Dr. Andrew Ellington and his team have identified and engineered a novel group II intron reverse transcriptase, RT-12. Dr. Ellington is a Professor in Molecular Biosciences, the Nancy Lee and Perry R. Bass Regents Chair in Molecular Biology, and holds the Wilson M. and Kathryn Fraser Research Professorship in Biochemistry at UT Austin. His...
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Low-cost rapid diagnosis of viral diseases
Background/problem
Detection of infectious diseases relies on amplification techniques of genetic material such as RNA or DNA. State-of-the-art amplification techniques include RT-PCR and RT-LAMP, both of which use a reverse transcriptase (RT) to amplify RNA. Usually, an RNA strand is reverse transcribed into complementary DNA (cDNA), which is...
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