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Allosteric transcription factors as peptide sensors
Modular intracellular peptide sensors for programmable gene control
Background
Synthetic biology increasingly relies on precise gene regulation systems that can sense and respond to specific molecular signals inside living cells. Peptides and peptide-tagged proteins are especially important targets because they govern communication, signaling, and...
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Supercharged fluorescent protein biosensors for real-time lanthanide monitoring
Background
Rare earth elements—particularly lanthanides like terbium, thulium, and dysprosium—are essential to technologies underpinning renewable energy, electrified transportation, high-performance magnets, and medical imaging. As demand accelerates, sustainable extraction and recycling processes require precise monitoring of these valuable...
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Sequence-defined oligourethane probe libraries for high-resolution surface characterization
Background
Understanding the chemical landscape of material and biological surfaces at the molecular level is critical for advancing applications in materials science, biotechnology, and environmental monitoring. Surface-bound functional groups govern key interactions relevant to contamination detection, catalyst performance, biosensing, and drug discovery....
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DNAzyme-based biosensor technology for selective and real-time detection of potassium ions in biological systems
Background
The ability to monitor potassium ion (K+) dynamics is of critical importance, particularly in the context of cancer research and neurobiology. Potassium ions play a fundamental role in maintaining cellular homeostasis, regulating membrane potential, and influencing processes such as cell proliferation, apoptosis, and immune responses. Disruptions...
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CRISPR-enhanced biosensor for ultrasensitive and multiplexed enzyme activity sensing
Background
Proteases play essential roles in numerous biological and pathological processes, including inflammation, cancer metastasis, neurodegeneration, and infectious disease. Specific protease activity serves as a valuable biomarker for disease progression, treatment monitoring, and drug development. However, existing detection methods are often...
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DNAzyme-seq: a single-cell metal ion and transcriptome profiling platform
Background
Metal ions such as zinc, copper, and calcium play indispensable roles in nearly every facet of cellular physiology, from enzymatic catalysis to signal transduction. However, conventional single-cell transcriptomics has largely overlooked the contribution of metal cofactors to gene expression, metabolism, and disease progression....
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Engineered E. coli platform for high-yield selenoprotein expression
Background
Selenoproteins, which incorporate the amino acid selenocysteine (Sec), are prized for their unique redox properties and catalytic capabilities in therapeutic antibodies, industrial enzymes, and diagnostic reagents. However, these proteins remain difficult and costly to produce in large quantities. Traditional chemical synthesis is labor-intensive...
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Engineered bacterial platform for in situ mRNA delivery to mammalian cells
Background
Genetic medicine depends on the ability to deliver messenger RNA (mRNA) into mammalian cells to enable therapeutic protein expression, correct genetic defects, or produce antigens for vaccines. But current delivery systems face critical barriers that limit their clinical utility. DNA plasmid-based approaches often show poor transfection...
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Engineered microbial antigen-presenting system for targeted T cell activation
Background
Targeted immunotherapy depends on the ability to modulate specific T cell populations to fight chronic infections, cancers, and autoimmune diseases. Achieving precise control over T cell activation is essential for generating effective immune responses while minimizing off-target effects. Traditional vaccines and existing antigen-presenting...
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One-pot assembly of long, modified DNA via scaffolded oligo ligation
Background
As synthetic biology advances, the need for efficient construction of long, chemically modified DNA has become increasingly critical across applications such as gene editing, DNA data storage, epigenetics, and personalized therapeutics. These technologies require precise, site-specific incorporation of chemical tags into DNA strands that...
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