Search results for Life+sciences+%3e+Research+tools+%3e+Reagent

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Low-cost rapid diagnosis of viral diseases
Background/problem Detection of infectious diseases relies on amplification techniques of genetic material such as RNA or DNA. State-of-the-art amplification techniques include RT-PCR and RT-LAMP, both of which use a reverse transcriptase (RT) to amplify RNA. Usually, an RNA strand is reverse transcribed into comple­mentary DNA (cDNA), which is...
Innovative modularization of One Pot PURE system for commercial unnatural amino acid incorporation
Background The integration of non-canonical amino acids into proteins holds immense promise for various industries, from biomedicine to biotechnology. However, traditional methods face challenges like high costs and batch variability. The One Pot PURE system, a streamlined version of cell-free protein synthesis, addresses these issues, offering precise...
Thermostable reverse transcriptase based on a thermostable DNA polymerase
Background Reverse transcriptase enzymes are pivotal in molecular biology for transcribing RNA into DNA, facilitating studies from gene expression analysis to viral detection. The reliability and efficiency of reverse transcription are crucial for accurate cDNA formation, a foundational step necessary for subsequent applications such as cloning and...
SuperFi high-fidelity Cas9 enzyme eliminates off‑target CRISPR cleavage
Background The widespread use of CRISPR/Cas9 as a programmable genome editing tool has encountered hurdles, due to concerns about potential off‑target DNA cleavage by Cas9. While the analysis of off-target editing events has led to the develop­ment of Cas9 variants with greater sensitivity to mismatches, the underlying molecular mechanisms by which...
Plastic-degrading enzymes
Able to break down PET plastics in as little as 24 hours Overview Plastic waste poses an ecological challenge. There have been 8.3 billion metric tons of plastics produced—6 billion tons are waste. What do we do with this waste? The long-term, sustainable solution is to find effective ways to break down the plastic into smaller parts and then...
Engineered methyltransferase for microbial production of the Alzheimer’s drug galantamine
­Problem Galantamine is a cholinesterase-inhibiting compound used for the treatment of cognitive decline in mild to moderate cases of Alzheimer’s disease and other memory impairment disorders. Galantamine is a plant alkaloid found in the bulbs and flowers of several species including daffodils, snowdrops, and red spider lilies. Galantamine...
An engineered methyltransferase enzyme for improved production of benzylisoquinoline alkaloids
Background Tetrahydropapaverine (THP) and papaverine are naturally occurring plant alkaloids that are commercially important. THP is a precursor of the drugs atracurium and cisatracurium, used as muscle relaxants. Papaverine is used as an antispasmodic. These clinically useful molecules may be synthesized using traditional organic synthesis methods...
Bacterial reagents: stable, low-cost, readily available reagents with no purification requirements
Problem Most molecular biology techniques commonly used in research, biotechnology, healthcare, and education rely heavily on purified functional protein reagents. However, purification of these protein reagents requires substantial investment of time, expertise, equipment, and infrastructure. As a result, this significantly limits the availability...
Br512: an improved DNA polymerase for isothermal amplification assays
Unmet need Loop-mediated isothermal amplification (LAMP) is a technique that allows for nucleic acid amplification without the need for a thermocycler. This technology enables testing for infections practically anywhere. Testing shortages have exacerbated the COVID-19 pandemic, and there is a high demand for tests that can provide rapid results outside...
A novel polymer for the quantitative cryopreservation of cells and tissue
Technology summary Cryopreservation science has seen only limited progress in recent decades. The discovery and development of a non-toxic, universal approach to complex cell and tissue cryopreservation could transform medicine through preservation of cell therapy products, organ banking, and lead to advancements that would decrease mortality and improve...