|
DNAzyme-seq: a single-cell metal ion and transcriptome profiling platform
Background
Metal ions such as zinc, copper, and calcium play indispensable roles in nearly every facet of cellular physiology, from enzymatic catalysis to signal transduction. However, conventional single-cell transcriptomics has largely overlooked the contribution of metal cofactors to gene expression, metabolism, and disease progression....
|
|
DNA-based sensors for live-cell mapping of metabolite- and metal ion-protein interactions
Background
Understanding how metabolites and metal ions interact with proteins inside living cells is essential for decoding cellular signaling, metabolic regulation, and disease mechanisms. These small molecules function as cofactors, second messengers, and allosteric regulators, orchestrating critical pathways that govern cell behavior. However,...
|
|
Thermotargetron for modifying thermophilic microorganisms
The Thermotargetron is a genetic tool for modifying thermophilic microorganisms using a type-II intron element, TeI3c‑4c. It allows efficient gene inactivation across a broad host range without needing selection markers and can target short gene sequences.
Background
Renewable fuels like bioethanol are increasingly vital due to rising global...
|
|
Advanced techniques for generating antigen-specific antibodies
Background
Biotechnology has advanced in the development and application of antibodies for therapeutic, diagnostic, and research purposes. Antibodies are crucial for their ability to specifically bind to antigens, making them valuable tools in the treatment of diseases, including cancer, autoimmune disorders, and infectious diseases. However, the identification...
|
